Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

lambda Phage cro repressor interaction with DNA.

K T Arndt, F Boschelli, J Cook

    The Journal of Biological Chemistry
    |April 10, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Global Bioequivalence Harmonisation Initiative (GBHI): Report of the sixth international EUFEPS/PQRI conference.

    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
    Same author

    Estimating the Age of Disease-causal HPV Infection Based on the Natural History of CIN2+ Among Females in Canada.

    Open forum infectious diseases·2025
    Same author

    Psychosocial outcomes after varying risk management strategies in women at increased familial breast cancer risk: a mixed methods study of patient and partner outcomes.

    Annals of the Royal College of Surgeons of England·2023
    Same author

    Drug Permeability - Best Practices for Biopharmaceutics Classification System (BCS)-Based Biowaivers: A workshop Summary Report.

    Journal of pharmaceutical sciences·2023
    Same author

    Analysis of rare disruptive germline mutations in 2135 enriched BRCA-negative breast cancers excludes additional high-impact susceptibility genes.

    Annals of oncology : official journal of the European Society for Medical Oncology·2022
    Same author

    Corrigenda.

    The bone & joint journal·2021

    This study corrects previous errors in assigning NMR signals for the cro repressor protein from phage lambda. It demonstrates a new method using fluorinated amino acids for better structural analysis of protein-DNA interactions.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Structural Biology

    Background:

    • The cro repressor protein from Escherichia coli lysogenic phage lambda plays a crucial role in viral DNA replication.
    • Accurate structural and functional analysis of the cro repressor is essential for understanding phage lambda biology.
    • Previous resonance assignments for the cro repressor's aromatic region using conventional methods contained errors.

    Purpose of the Study:

    • To achieve complete and accurate identification of resonances in the aromatic region of the 1H NMR spectrum of the cro repressor.
    • To investigate the effects of DNA complex formation on the cro repressor structure using NMR spectroscopy.
    • To validate and refine existing models of cro repressor-DNA complexes.

    Main Methods:

    • Utilized two-dimensional NMR analysis for resonance identification.

    Related Experiment Videos

  • Employed specifically deuterated tyrosines to aid spectral assignment.
  • Incorporated 3-fluorophenylalanine or 3-fluorotyrosine into the cro repressor for 19F NMR studies.
  • Used a cloned structural gene in an inducible system for producing modified proteins.

    • Main Results:

    • Successfully identified resonances in the aromatic region of the 1H NMR spectrum of the cro repressor.
    • Revealed substantial errors in previously published resonance assignments.
    • Demonstrated that fluoro-analogues are practical for NMR spectroscopy, enabling specific nuclear spin incorporation.
    • Observed changes in NMR spectra upon complex formation with DNA, providing insights into structural alterations.

    Conclusions:

    • The study provides a complete and accurate resonance assignment for the cro repressor's aromatic region.
    • The findings correct significant errors in prior research, improving the reliability of cro repressor structural data.
    • The use of fluorinated analogues and inducible gene expression systems is a viable strategy for producing protein samples for advanced NMR studies.
    • The NMR data offer direct experimental support for speculative models of cro repressor-DNA complexes derived from X-ray crystallography.