Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutations affecting two different steps in transcription initiation at the phage lambda PRM promoter.

M C Shih, G N Gussin

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1983
    PubMed
    Summary

    Mutations in phage lambda's P(RM) promoter affect transcription initiation. Some mutations impact RNA polymerase binding, while others affect the transition to open complexes, revealing promoter region roles.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Two-dimensional electronic transport and surface electron accumulation in MoS<sub>2</sub>.

    Nature communications·2018
    Same author

    Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2013
    Same author

    In vitro oxidation of MPTP by primate neural tissue: A potential model of MPTP neurotoxicity.

    Neurochemistry international·2010
    Same author

    Dopamine transporter regulation during four nights of REM sleep deprivation followed by recovery--an in vivo molecular imaging study in humans.

    Sleep·2010
    Same author

    Intact ribonucleic acid from defective particles of bacteriophage R17.

    Journal of molecular biology·2009
    Same author

    Three complementation groups in bacteriophage R17.

    Journal of molecular biology·2009

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • The P(RM) promoter of phage lambda is crucial for regulating viral gene expression.
    • Understanding promoter function requires dissecting the roles of specific DNA sequences in transcription initiation.

    Purpose of the Study:

    • To investigate the impact of specific mutations on the activity of the phage lambda P(RM) promoter.
    • To elucidate the roles of different promoter regions in RNA polymerase binding and transcription initiation.

    Main Methods:

    • Utilized the abortive initiation assay to study promoter activity in vitro.
    • Measured kinetic parameters: k(2) (isomerization rate) and K(B) (binding equilibrium).
    • Compared wild-type P(RM) with four mutant promoters in the presence and absence of repressor.

    Related Experiment Videos

    Main Results:

    • Repressor significantly stimulated the isomerization rate (k(2)) of the wild-type promoter.
    • Mutations at nucleotides -33 and -38 (prmU31, prmE104) reduced RNA polymerase binding (K(B)) without affecting isomerization (k(2)).
    • A mutation at nucleotide -14 (prmE37) reduced isomerization (k(2)) but not binding (K(B)).
    • A mutation at nucleotide -39 (prmE93) disrupted repressor binding to the O(B)2 operator site.

    Conclusions:

    • The -35 and -10 regions of Escherichia coli promoters may independently influence distinct steps in transcription initiation.
    • Specific promoter DNA sequences differentially affect RNA polymerase binding and the transition to the open complex.
    • Mutations can selectively impair either polymerase binding or the isomerization step of transcription initiation.