Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Role of gene T in phage lambda tail assembly.

L C Tsui, R W Hendrix

    Virology
    |March 1, 1983
    PubMed
    Summary

    The bacteriophage lambda T gene is crucial for tail assembly, with mutations affecting tail protein activity and virion stability. This research clarifies the gene T product's role in the tail assembly pathway.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cloning of a DSS1 pseudogene (DSS1P1) and mapping to human Chromosome band 5ql4.

    Mammalian genome : official journal of the International Mammalian Genome Society·2016
    Same author

    Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses.

    Environmental microbiology·2012
    Same author

    Jumbo bacteriophages.

    Current topics in microbiology and immunology·2009
    Same author

    Crosslinking in viral capsids via tiling theory.

    Journal of theoretical biology·2005
    Same author

    Where are the pseudogenes in bacterial genomes?

    Trends in microbiology·2002
    Same author

    Spectrum of mutations in the CFTR gene of patients with classical and atypical forms of cystic fibrosis from southwestern Sweden: identification of 12 novel mutations.

    Genetic testing·2002

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage lambda tail assembly is a complex process involving multiple genes and proteins.
    • Heat-sensitive mutations provide valuable tools for dissecting essential gene functions.

    Purpose of the Study:

    • To investigate the phenotypic effects of a heat-sensitive mutation in bacteriophage lambda tail gene T.
    • To determine the precise role of gene T in the lambda tail assembly pathway.

    Main Methods:

    • Analysis of lambda Tts40 prophage induction and growth at permissive and non-permissive temperatures.
    • In vitro complementation assays using bacterial lysates.
    • Examination of phage particle morphology and protein cleavage (gpH).
    • Heat inactivation assays of purified virions.

    Main Results:

    • Lambda Tts40 prophage produced morphologically normal but inactive tails at non-permissive temperatures, requiring gene products T, U, and Z for in vitro activation.
    • Gene T acts early in tail assembly, before or during tail shaft polymerization, and its product is present in the virion.
    • Mutant virions showed heat sensitivity, indicating the T gene product's role in virion stability.

    Conclusions:

    • The bacteriophage lambda T gene product is essential for functional tail assembly and virion stability.
    • Gene T functions at a specific point in the tail assembly pathway, influencing tail shaft completion and protein processing.
    • The T gene product's presence in the virion contributes to its structural integrity and resistance to heat inactivation.

    Related Experiment Videos