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Related Experiment Videos

Con A suppressor cell assay: a further characterization.

P L Meroni, G Balestrieri, W Barcellini

    Journal of Clinical & Laboratory Immunology
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Concanavalin A (Con A) activated cells block immune enhancement rather than suppress responses. This Con A suppressor cell activity requires cell proliferation and monocyte cooperation, with reduced function observed in active lupus erythematosus patients.

    Area of Science:

    • Immunology
    • Cellular Immunology

    Background:

    • The Concanavalin A (Con A) induced suppressor cell assay is a method to study immune regulation.
    • Previous studies have explored the mechanisms of Con A-induced suppression.

    Purpose of the Study:

    • To further characterize the Con A induced suppressor cell assay.
    • To investigate the nature of Con A-activated cells' effect on immune responses.
    • To explore the role of monocytes and cell proliferation in Con A suppressor cell activity.

    Main Methods:

    • Coculture of autologous fresh cells with Con A-activated cells.
    • Comparison of mitogenic responses with control cell cocultures.
    • Monocyte depletion experiments.
    • Assessment of cytotoxicity.

    Related Experiment Videos

  • Analysis of mononuclear cells from active and inactive Systemic Lupus Erythematosus (SLE) patients.

    • Main Results:

    • Con A-activated cells reduced mitogenic responses compared to control cells.
    • Con A-activated cells did not induce true suppression, but blocked enhancement.
    • Con A suppressor cell activity required cell proliferation but was not proportional to DNA synthesis.
    • Monocyte-depleted populations showed reduced Con A suppressor cell activity.
    • Activity was not cytotoxic and depended on the number of activated cells.
    • Active SLE patients showed a significant loss of the suppression index compared to controls.

    Conclusions:

    • Con A activation primarily blocks immune enhancement rather than inducing direct suppression.
    • Monocytes play a cooperative role in the induction of Con A suppressor cell activity.
    • Impaired Con A suppressor cell activity is associated with active Systemic Lupus Erythematosus (SLE).