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Related Experiment Videos

Experimental bacteriophage set for typing Staphylococcus intermedius.

J Kawano, A Shimizu, S Kimura

    Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie = International Journal of Microbiology and Hygiene. A, Medical Microbiology, Infectious
    |December 1, 1982
    PubMed
    Summary

    A novel set of 14 phages effectively types Staphylococcus intermedius strains from various animals. This phage typing method demonstrates high efficacy for identifying different ecovars of this bacterium.

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    Area of Science:

    • Veterinary Microbiology
    • Bacteriology
    • Phage Typing

    Background:

    • Staphylococcus intermedius is a significant pathogen in companion and domestic animals.
    • Accurate identification and characterization of S. intermedius strains are crucial for epidemiological studies and disease control.
    • Existing phage typing methods may lack specificity or coverage for S. intermedius ecovars.

    Purpose of the Study:

    • To develop and evaluate a new phage set for the reliable typing of Staphylococcus intermedius strains.
    • To assess the efficacy of the proposed phage set across different animal-derived S. intermedius ecovars.

    Main Methods:

    • A set of 14 phages was utilized for phage typing.
    • 581 S. intermedius strains were isolated from dogs, pigeons, horses, foxes, and mink.

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  • Strains were subjected to typing at routine test dilution (RTD) and 100 times RTD.

    • Main Results:

    • Typability of S. intermedius ecovars ranged from 78.9% to 100%.
    • The majority of strains were successfully typed at RTD.
    • Four distinct phage patterns were identified: Canine I, Canine II, Pigeon, and Equine.

    Conclusions:

    • The proposed phage set is a valuable tool for differentiating Staphylococcus intermedius strains.
    • This new typing system offers improved characterization of S. intermedius from diverse animal reservoirs.
    • The phage set demonstrates high utility for epidemiological surveillance of S. intermedius.