Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocytes with parallel tubular structures: morphologically a distinctive subpopulation.

J W Smit, N R Blom, M van Luyn

    Blut
    |June 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Rare NOX3 Variants Confer Susceptibility to Agranulocytosis During Thyrostatic Treatment of Graves' Disease.

    Clinical pharmacology and therapeutics·2017
    Same author

    Circulating long-non coding RNAs as biomarkers of left ventricular diastolic function and remodelling in patients with well-controlled type 2 diabetes.

    Scientific reports·2016
    Same author

    Postural orthostatic tachycardia is not a useful diagnostic marker for chronic fatigue syndrome.

    Journal of internal medicine·2016
    Same author

    Vascular Health in Patients in Remission of Cushing's Syndrome Is Comparable With That in BMI-Matched Controls.

    The Journal of clinical endocrinology and metabolism·2016
    Same author

    6-Mercaptopurine, Still Valuable for the Palliative Treatment of Acute Myeloid Leukaemia.

    Hematology (Amsterdam, Netherlands)·2016
    Same author

    Persistent self-consciousness about facial appearance, measured with the Derriford appearance scale 59, in patients after long-term biochemical remission of acromegaly.

    Pituitary·2014
    Same journal

    Clostridial septicaemia with intense hemolysis complicating septic abortion.

    Blut·1990
    Same journal

    Improvement of pneumonia and arthritis in Felty's syndrome by treatment with granulocyte-macrophage colony-stimulating factor (GM-CSF).

    Blut·1990
    Same journal

    Flow cytometric analysis of tissue factor (TF) expression on stimulated monocytes--comparison to procoagulant activity of mononuclear blood cells.

    Blut·1990
    Same journal

    Coagulopathy after snake bite by Bothrops neuwiedi: case report and results of in vitro experiments.

    Blut·1990
    Same journal

    Septicemia due to Streptococcus mitis in neutropenic patients with acute leukemia.

    Blut·1990
    Same journal

    Variations in erythropoiesis throughout a lifetime. Studies in a high-leukaemic mouse strain, the AKR/O strain, and a non-leukaemic strain, the WLO strain.

    Blut·1990
    See all related articles

    This study reveals two distinct T cell subsets in peripheral blood, one with unique granules and tubular structures. These Fc gamma receptor-bearing lymphocytes represent a morphologically distinct subpopulation.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Lymphocyte subpopulations play critical roles in immune responses.
    • Morphological characterization aids in understanding cell function and differentiation.
    • Fc gamma receptors are key markers for certain lymphocyte subsets.

    Purpose of the Study:

    • To investigate the ultrastructural features of fractionated peripheral blood lymphocyte subpopulations.
    • To identify distinct morphological characteristics within T, B, and Null cell enriched fractions.
    • To correlate specific ultrastructural features with Fc gamma receptor-bearing lymphocytes.

    Main Methods:

    • Fractionation of peripheral blood lymphocytes into T, B, and Null cell subsets using sheep red blood cell rosettes and nylon wool adherence.

    Related Experiment Videos

  • Ultrastructural analysis of isolated lymphocyte subpopulations.
  • Lysis of sheep erythrocytes from rosette-forming cells to obtain pure T cell fractions.
  • Mechanical vibration of rosette-forming cells as an alternative T cell isolation method.

    • Main Results:

    • Two distinct T cell subsets were identified: a majority with smooth membranes and a minority (18%) with rough membranes, amorphous granules, and parallel tubular structures (p.t.s.).
    • Mechanical vibration of T cells yielded a population predominantly matching the rough membrane, granular subset.
    • Cells with p.t.s. and amorphous granules were found in Null (50%) and B cell (25%) enriched fractions, likely representing contaminating Null cells.

    Conclusions:

    • Peripheral blood T lymphocytes comprise at least two morphologically distinct subsets.
    • The presence of amorphous granules and parallel tubular structures (p.t.s.) characterizes a specific lymphocyte subpopulation.
    • Fc gamma receptor-bearing lymphocytes appear to form a morphologically distinct subset, characterized by these unique ultrastructural features.