Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The glucose effect in Bacillus subtilis.

V L Price, J A Gallant

    European Journal of Biochemistry
    |July 15, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Bacillus subtilis exhibits a

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Vaccines for parasitic diseases.

    Current drug targets. Infectious disorders·2002
    Same author

    Plasmodium falciparum: immunogenicity of alum-adsorbed clinical-grade TBV25-28, a yeast-secreted malaria transmission-blocking vaccine candidate.

    Experimental parasitology·2001
    Same author

    Ribosomes can slide over and beyond "hungry" codons, resuming protein chain elongation many nucleotides downstream.

    Proceedings of the National Academy of Sciences of the United States of America·1998
    Same author

    Expression and purification of correctly processed, active human TACE catalytic domain in Saccharomyces cerevisiae.

    Protein expression and purification·1998
    Same author

    Saccharomyces cerevisiae-secreted fusion proteins Pfs25 and Pfs28 elicit potent Plasmodium falciparum transmission-blocking antibodies in mice.

    Infection and immunity·1998
    Same author

    Inducible expression cassettes in yeast: ADH2.

    Methods in molecular biology (Clifton, N.J.)·1997
    Same journal

    Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

    European journal of biochemistry·2020
    Same journal

    AB 3.1.1.1 (or EC 3.1.1.?).

    European journal of biochemistry·2020
    Same journal

    Cdk5.

    European journal of biochemistry·2018
    Same journal

    Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

    European journal of biochemistry·2004
    Same journal

    Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

    European journal of biochemistry·2004
    Same journal

    Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

    European journal of biochemistry·2004
    See all related articles

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • The 'glucose effect' describes the catabolite repression of alternative carbon source utilization by glucose in bacteria.
    • Understanding glucose metabolism regulation is crucial for microbial physiology and biotechnology.

    Purpose of the Study:

    • To elucidate the molecular mechanisms underlying the glucose downshift response in Bacillus subtilis.
    • To identify key enzymes regulated during the transition from glucose metabolism.

    Main Methods:

    • Enzyme activity assays were performed.
    • Analysis of enzyme levels and modifications was conducted.
    • Investigated the role of glucose transport in enzyme regulation.

    Main Results:

    Related Experiment Videos

    • Identified the depression of key catabolic enzymes, including isocitrate dehydrogenase and glucose-6-phosphate dehydrogenase, during glucose downshift.
    • Observed a reversible modification of phosphofructokinase, linked to glucose transport.

    Conclusions:

    • The glucose downshift mechanism in Bacillus subtilis involves the coordinated regulation of specific catabolic enzymes.
    • Glucose transport plays a regulatory role in enzyme modification, impacting metabolic pathways.