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Pregnenolone and its sulfate ester in the rat brain
Brain Research
|June 27, 1983
Summary
Pregnenolone (P) and its sulfate ester (PS) are found in high concentrations in the rat brain, exceeding plasma levels. Brain P and PS levels are independent of peripheral endocrine glands, suggesting local synthesis.
Area of Science:
- Neuroendocrinology
- Steroid Biochemistry
Background:
- Pregnenolone (P) and its sulfate ester (PS) are neurosteroids with known functions in the brain.
- Previous studies have characterized dehydroepiandrosterone sulfate (DS) in the rat brain.
Purpose of the Study:
- To characterize the concentrations of pregnenolone (P) and its sulfate ester (PS) in the adult male rat brain.
- To investigate the potential influence of peripheral endocrine glands on brain P and PS levels.
Main Methods:
- Quantification of P and PS concentrations in anterior and posterior brain regions of adult male rats.
- Comparison of brain P and PS levels with those in plasma.
- Assessment of P and PS levels following adrenalectomy and orchiectomy compared to sham-operated controls.
Main Results:
- Brain concentrations of P and PS significantly exceeded those found in plasma.
- P concentrations in the brain were substantially higher than DS concentrations.
- No significant differences in brain P and PS levels were observed after adrenalectomy plus orchiectomy, indicating independence from peripheral steroidogenic glands.
Conclusions:
- The rat brain contains significant levels of pregnenolone (P) and its sulfate ester (PS).
- Brain P and PS levels appear to be regulated by mechanisms within the brain itself, independent of the peripheral endocrine system.
- These findings suggest local synthesis or accumulation of P and PS in the brain.