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Related Experiment Videos

Bioassay procedures for norfloxacin.

J Bland, A Edison, J Huber

    European Journal of Clinical Microbiology
    |June 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A new bioassay accurately quantifies norfloxacin in biological samples like serum, tissue, and urine. This method offers a reliable alternative for measuring norfloxacin concentrations using Klebsiella pneumoniae.

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    Area of Science:

    • Microbiology
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Accurate quantification of antibiotics is crucial for therapeutic drug monitoring and pharmacokinetic studies.
    • Existing methods for norfloxacin quantification may require specialized equipment or extensive sample preparation.
    • Development of a sensitive and accessible bioassay can improve drug monitoring capabilities.

    Purpose of the Study:

    • To develop and validate a disk-diffusion bioassay for the quantitative determination of norfloxacin.
    • To assess the assay's performance in biological matrices including serum, tissue, and urine.
    • To establish the assay's sensitivity and comparability with established analytical techniques.

    Main Methods:

    • A disk-diffusion bioassay was established using Klebsiella pneumoniae ATCC 10031 as the indicator organism.

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  • Norfloxacin concentrations were determined by measuring zones of inhibition, with options for manual or automated analysis.
  • Urine samples were pre-treated with a 1:2 dilution in 0.1N HCl to ensure norfloxacin solubility.

    • Main Results:

    • The developed bioassay demonstrated a sensitivity of 0.2 microgram/ml when incubated at 28 degrees C.
    • Measurement of inhibition zones could be performed using standard laboratory tools or automated image analysis.
    • Norfloxacin concentrations determined by bioassay in urine samples showed good agreement with high-performance liquid chromatography (HPLC) results.

    Conclusions:

    • A robust disk-diffusion bioassay for norfloxacin quantitation in serum, tissue, and urine has been successfully developed.
    • The assay is sensitive and offers flexibility in measurement techniques.
    • The bioassay provides comparable results to HPLC, suggesting its utility as a reliable analytical tool.