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Partial characterization of immunosuppressive factors from short-term cultures of murine decidual tissue
Previous in vitro experiments provided evidence for the immunosuppressive properties of supernatants from short-term cultures of decidual tissue obtained from the uterus of the pregnant mouse. The inhibitory activity of supernatants, previously detected in a mixed lymphocyte reaction (MLR), has now been demonstrated in an in vitro thymocyte proliferation assay. Fractionation of these supernatants on Sephadex G-15 and Sephacryl S-300 revealed that inhibition of the thymocyte proliferation assay was associated only with a low molecular weight fraction (less than 1,500), whilst MLR inhibitory activity was associated with three different molecular weight fractions (less than 1,500, 60,000 and 1,000,000). These results are discussed in relation to the different biological factors previously isolated in decidua or deciduoma of rats and humans.
Previous in vitro experiments provided evidence for the immunosuppressive properties of supernatants from short-term cultures of decidual tissue obtained from the uterus of the pregnant mouse. The inhibitory activity of supernatants, previously detected in a mixed lymphocyte reaction (MLR), has now been demonstrated in an in vitro thymocyte proliferation assay. Fractionation of these supernatants on Sephadex G-15 and Sephacryl S-300 revealed that inhibition of the thymocyte proliferation assay was associated only with a low molecular weight fraction (less than 1,500), whilst MLR inhibitory activity was associated with three different molecular weight fractions (less than 1,500, 60,000 and 1,000,000). These results are discussed in relation to the different biological factors previously isolated in decidua or deciduoma of rats and humans.