Simultaneous analysis of 5'-deoxy-5-fluorouridine and 5-fluorouracil in plasma by analytical isotachophoresis

Analytical isotachophoresis was used for the determination of 5-fluorouracil and 5'-deoxy-5-fluorouridine in plasma. The inclusion of spacers in the system greatly improved the separation and quantitation. The method can be employed for simultaneous measurements of fluorinated pyrimidines used in clinical practice.