High-performance liquid chromatographic analysis of iodochlorhydroxyquin and hydrocortisone in ointments and creams

A simple isocratic, high-performance liquid chromatographic (HPLC) assay procedure was developed for the simultaneous determination of iodochlorhydroxyquin and hydrocortisone in ointments and creams using phenyl salicylate as an internal standard. Ointment samples were extracted by direct dissolution in ether. Homogeneous suspensions of the creams were prepared in the mobile phase. The samples were spiked by the addition of standard iodochlorhydroxyquin, standard hydrocortisone, and the internal standard and subsequently extracted with the mobile phase. HPLC was performed using a reverse-phase microparticulate C-18 column, a precolumn, and a UV detector set at 256 nm. A mobile phase containing methanol and 0.05 M phosphoric acid (70:30) was employed at a flow rate of 1 ml/min. The percent iodochlorhydroxyquin and hydrocortisone found to be present in eight commercial products is reported.