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Lambda phage DNA sequences affecting the packaging process.

T Miwa, K Matsubara

    Gene
    |October 1, 1983
    PubMed
    Summary
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    Bacteriophage lambda DNA packaging requires a minimal region and an adjacent enhancing region containing a terminase binding site. This enhancing region stimulates DNA cutting and packaging when physically linked to the minimal region.

    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Bacteriophage lambda DNA packaging is a complex process involving specific DNA recognition sites.
    • Previous research identified a minimal DNA region essential for lambda phage packaging.

    Purpose of the Study:

    • To investigate the role of an adjacent DNA region in bacteriophage lambda packaging.
    • To characterize the function and sequence elements of the enhancing region for packaging.

    Main Methods:

    • Analysis of DNA sequences involved in bacteriophage lambda packaging.
    • Identification of binding sites for lambda terminase (Nu1 and A gene products).
    • Comparative sequence analysis with related bacteriophages (e.g., phi 80).

    Main Results:

    Related Experiment Videos

    • A minimal packaging region (40 bp right arm, 45 bp left arm) and a cos lambda cutting site (22 bp right arm, 38 bp left arm) were defined.
    • An 84-bp enhancing region, containing a lambda terminase binding site, stimulates cos lambda cutting and packaging.
    • The enhancing region's activity depends on its proximity to the minimal packaging region.
    • The enhancing region contains 15-bp inverted repeats conserved in bacteriophage phi 80.

    Conclusions:

    • The enhancing region, containing a terminase binding site, plays a crucial role in stimulating bacteriophage lambda DNA packaging.
    • Cooperation between the minimal packaging region and the enhancing region is essential for efficient packaging.
    • Sequence conservation in the enhancing region suggests a conserved mechanism for DNA packaging in related phages.