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Effect of Con A stimulation on antibody dependent cell-mediated cytotoxicity of human lymphocyte subpopulations
Con A activated human peripheral blood lymphocytes and their subpopulations: T, Ty-, Ty+, and non-T cells were studied for their ability to mediate antibody-dependent cell mediated cytotoxicity (ADCC) to chick erythrocytes coated with rabbit IgG antibody. Ficoll-Hypaque separated lymphocytes increased their cytotoxic activity following 14 h activation with Con A. T lymphocytes mainly appeared to be responsible for this effect. The increase of cytotoxic in ADCC was limited to Ty + cells. T lymphocytes deprived of Ty+ cells did not show cytotoxic effect in spite of stimulation with Con A and generation of new T cells with FcIgG receptors. The two-fold increase in the number of FcIgG bearing lymphocytes following enzymatic treatment with pronase and neuraminidase did not augment their activity in ADCC system. Our results indicate that the increase of ADCC activity in T cell population stimulated with Con A depends on cells already expressing FcIgG receptors and not on the induction of new Ty+ cells with K lymphocyte activity.
Con A activated human peripheral blood lymphocytes and their subpopulations: T, Ty-, Ty+, and non-T cells were studied for their ability to mediate antibody-dependent cell mediated cytotoxicity (ADCC) to chick erythrocytes coated with rabbit IgG antibody. Ficoll-Hypaque separated lymphocytes increased their cytotoxic activity following 14 h activation with Con A. T lymphocytes mainly appeared to be responsible for this effect. The increase of cytotoxic in ADCC was limited to Ty + cells. T lymphocytes deprived of Ty+ cells did not show cytotoxic effect in spite of stimulation with Con A and generation of new T cells with FcIgG receptors. The two-fold increase in the number of FcIgG bearing lymphocytes following enzymatic treatment with pronase and neuraminidase did not augment their activity in ADCC system. Our results indicate that the increase of ADCC activity in T cell population stimulated with Con A depends on cells already expressing FcIgG receptors and not on the induction of new Ty+ cells with K lymphocyte activity.