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Isolation and nucleotide sequence of a partial cDNA clone for bovine opsin
Biochemical and Biophysical Research Communications
|October 31, 1983
Summary
Researchers cloned bovine complementary DNA (cDNA) to analyze the opsin gene. Southern blot analysis suggests the bovine opsin gene exists as a single copy per haploid genome.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Opsins are critical proteins in visual phototransduction.
- Understanding the genetic structure of opsin in bovine species is essential for visual research.
Purpose of the Study:
- To clone and characterize bovine complementary DNA (cDNA) encoding opsin.
- To determine the copy number of the bovine opsin gene within the genome.
Main Methods:
- Hybridization using synthetic deoxyribonucleotides to clone bovine cDNAs.
- Northern blot hybridization to determine opsin mRNA size.
- Southern blot hybridization to analyze bovine DNA and gene copy number.
Main Results:
- The longest cDNA clone, pBO-1, contained an 811-bp insert with coding and 3'-untranslated regions of opsin peptide.
- Opsin mRNA was identified as 23S in size.
- Southern blot analysis indicated a single band for the bovine opsin gene with Eco RI, Hind III, and Bam HI restriction enzymes.
Conclusions:
- The bovine opsin gene sequence was successfully cloned and characterized.
- Evidence suggests the bovine opsin gene is present as a single copy per haploid genome.