Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alkylating agent interactions with the nuclear matrix.

K D Tew, A L Wang, P S Schein

    Biochemical Pharmacology
    |December 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Mediating effect of social support and family function on mental health of women infected with HIV].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
    Same author

    [Analysis on negative conversion of syphilis-specific antibody and related factors in children born to pregnant women with syphilis in China, 2021].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
    Same author

    [Influencing factors and predictive model development for contralateral central lymph node metastasis in unilateral papillary thyroid microcarcinoma].

    Zhonghua yi xue za zhi·2025
    Same author

    [Analysis on rate of adverse pregnancy outcomes in HIV-infected pregnant women and influencing factors in some regions of China, 2017-2023].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
    Same author

    [Analysis of loss to follow-up status and influencing factors of children born to pregnant women with HIV infection in China in 2019].

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
    Same author

    [Epidemiological analysis of the current prevalence of hepatitis B virus infection among pregnant and postpartum women in China from 2021 to 2023].

    Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024
    Same journal

    Methylated SNHG3 lncRNA counteracts PSMD4-inhibited angiogenesis by HnRNPA2B1 SUMOylation in peripheral arterial disease.

    Biochemical pharmacology·2026
    Same journal

    The organelle-targeting duality of nanomaterials: physicochemical property-guided therapeutic effects and toxicological outcomes.

    Biochemical pharmacology·2026
    Same journal

    The central role of endoplasmic reticulum stress in Parkinson's disease and targeted therapeutic strategies.

    Biochemical pharmacology·2026
    Same journal

    Mechanisms involved in the regulation of carbohydrate and lipid metabolism by oleanane-type pentacyclic triterpenes.

    Biochemical pharmacology·2026
    Same journal

    Myricitrin alleviates paraquat-induced acute lung injury via PERK pathway to attenuate endoplasmic reticulum stress and mitochondrial dysfunction.

    Biochemical pharmacology·2026
    Same journal

    Design, synthesis, and biological evaluation of Combretastatin A4 dimers for the treatment of hepatocellular carcinoma.

    Biochemical pharmacology·2026
    See all related articles

    Nitrosourea drugs like chlorozotocin (CLZ) and CCNU damage DNA, reducing its binding to the nuclear matrix. This DNA-matrix disruption may explain the cytotoxic effects of these antineoplastic agents.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Cancer Research

    Background:

    • The nuclear matrix plays a crucial role in DNA organization, replication, and chromatin structure.
    • Nitrosourea and nitrogen mustard compounds are antineoplastic agents that interact with cellular components.

    Purpose of the Study:

    • To investigate the interaction of chlorozotocin (CLZ) and 1-(2-chloroethyl-3-cyclohexyl)-1-nitrosourea (CCNU) with the nuclear matrix in HeLa cells.
    • To determine the impact of DNA and nuclear matrix alkylation on DNA-matrix reassociation.

    Main Methods:

    • HeLa cells were treated with CLZ and CCNU to measure drug alkylation and carbamoylation of nuclear matrix components (matricin and RNP).
    • In vitro studies assessed the effect of DNA and matrix protein alkylation on DNA-matrix reassociation using controlled protein:DNA ratios.

    Related Experiment Videos

    Main Results:

    • CLZ and CCNU alkylated the nuclear matrix, with matricin components showing higher modification than RNP elements per microgram of protein.
    • Direct alkylation or carbamoylation of matrix proteins did not alter DNA-matrix binding.
    • Alkylation of DNA (1 alkylation/10(2) base pairs) significantly reduced DNA binding to the nuclear matrix by 60%.

    Conclusions:

    • DNA alkylation, rather than matrix protein modification, interferes with DNA-matrix association.
    • This disruption of DNA-matrix binding by antineoplastic agents may contribute to their cytotoxic effects.