Heterogeneity of Fc gamma receptor expression on human cell lines

Receptors for the Fc gamma portion of IgG (Fc gamma R) may be detected on several cell populations using a variety of assay techniques. The heterogeneity of these receptors on human cell lines has been investigated using a rabbit antiserum raised against Fc gamma R expressed on K562 cells. This reagent only partially inhibited Fc gamma R binding on K562 cells, but completely blocked that on Daudi cells. The results are compatible with the hypothesis that Daudi cells express only one form of Fc gamma R, which is similar if not identical to one of the 2 Fc gamma-binding activities detected on K562 cells. Cell lines may therefore be useful sources of different homogeneous Fc gamma R material for the development of reagents to study these receptors on cell subpopulations in health and disease.