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Glycoprotein I identification during normal and pathological megakaryocytic maturation
Blood Cells
|January 1, 1983
Summary
Glycoprotein I (GP I) is expressed throughout normal megakaryocyte (MK) maturation. Improved techniques detect GP I on early MK precursors, with increased expression in idiopathic thrombocytopenic purpura (ITP) and acute megakaryoblastic leukemia.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Glycoprotein I (GP I) is a marker on megakaryocyte (MK) precursors.
- Previous studies confirmed GP I expression during normal MK maturation.
Purpose of the Study:
- To investigate GP I expression on normal and pathological MK precursors.
- To evaluate the utility of monoclonal antibody AN51 for detecting GP I.
Main Methods:
- Immunofluorescence, immunogold, and immunoferritin electron microscopy were used.
- Monoclonal antibody AN51 was employed to detect GP I.
- Analysis was performed on normal MK precursors, bone marrow from ITP patients, and acute megakaryoblastic leukemia blasts.
Main Results:
- Improved immunofluorescence detected weak GP I labeling on early normal MK precursors (PMKB).
- Increased AN51-labeled PMKB were observed in ITP bone marrow.
- AN51 labeled PMKB in acute megakaryoblastic leukemia, but at a lower percentage than the J15 antibody.
Conclusions:
- GP I is expressed on early MK precursors, detectable with enhanced techniques.
- AN51 shows specificity for the MK lineage.
- AN51 requires combination with other MK markers for diagnosing acute megakaryoblastic leukemia due to weak PMKB labeling.