Localization of an intracellular membrane-bound Ca2+-ATPase in PtK-cells using immunofluorescence techniques

Monospecific antibodies to an intracellular membrane-bound Ca2+-ATPase were used to localize the enzyme in PtK-cells in interphase and in mitosis as well. In interphase the protein is distributed as small dots and rods in the cytoplasm with an increased concentration around the nucleus. Neither the plasma membrane nor the nuclear envelope are stained. In mitotic cells the Ca2+-ATPase is localized around the spindle rather than in it. The results are in agreement with the proposed function of enzyme as an essential part of the intracellular Ca2+-regulating system controlling Ca2+ in the respective domains of the cell.