Helix pomatia induced conversion of some 3 beta-hydroxysteroids

After incubating a dehydroepiandrosterone solution with an enzymatic preparation from Helix pomatia, the steroid recovery was markedly decreased. The transforming activity resulted specific for some 3 beta-hydroxysteroids and it appeared to lower after storing the preparation at 4 degrees C for some days. The presence of 4-androsten-3,17-dione within the incubation products suggests that the enzymatic preparation contains at least 3 beta-hydroxysteroid: NAD oxydoreductase and 3-oxosteroid-5-4-ene isomerase. The use of large amounts of freshly dissolved Helix pomatia juice solution may lead to unreliable results in steroid analyses.