Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SOS mutator effect in E. coli mutants deficient in mismatch correction.

P Caillet-Fauquet, G Maenhaut-Michel, M Radman

    The EMBO Journal
    |April 1, 1984
    PubMed
    Summary

    The SOS response in Escherichia coli, triggered by UV radiation, causes mutations. A key finding is that the methyl-directed mismatch correction system removes many of these mutations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genome-wide association analysis suggests novel loci for Hashimoto's thyroiditis.

    Journal of endocrinological investigation·2018
    Same author

    Longitudinal development of obesity in the post-Fontan population.

    European journal of clinical nutrition·2015
    Same author

    Toll-like receptor 4 plays significant roles during allergic rhinitis.

    Allergologia et immunopathologia·2014
    Same author

    [How much do we know about human brucellosis?].

    Zeitschrift fur Rheumatologie·2009
    Same author

    Acrylamide, an in vivo thyroid carcinogenic agent, induces DNA damage in rat thyroid cell lines and primary cultures.

    Molecular and cellular endocrinology·2006
    Same author

    Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line.

    Journal of virological methods·2004

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • The SOS response is a DNA damage response in bacteria.
    • UV irradiation induces the SOS response, which can lead to mutagenesis.
    • Bacteriophage lambda is a useful tool for studying bacterial genetics.

    Purpose of the Study:

    • To characterize the mutator effect of the SOS response in Escherichia coli.
    • To investigate the role of mismatch correction and DNA methylation in SOS-induced mutagenesis.
    • To understand the mechanisms underlying untargeted mutations during the SOS response.

    Main Methods:

    • Utilizing bacteriophage lambda to assess mutagenesis in Escherichia coli.
    • Measuring forward and reversion mutagenesis in specific phage genes.
    • Comparing SOS mutator effects in wild-type and mutant strains of E. coli (mutH, mutL, mutS, dam3).

    Main Results:

    • The SOS mutator effect was elevated in mismatch correction-deficient E. coli mutants (mutH, mutL, mutS).
    • The SOS mutator effect was reduced in an adenine methylation-deficient mutant (dam3).
    • A significant portion of SOS-induced untargeted mutations are corrected by the methyl-directed mismatch repair system.

    Conclusions:

    • The methyl-directed mismatch correction system plays a crucial role in removing SOS-induced untargeted mutations.
    • Reduced SOS mutator effects in dam mutants may result from selective chromosome killing during mismatch repair.
    • The SOS mutator effect on undamaged DNA involves decreased DNA synthesis fidelity.

    Related Experiment Videos