[Identification of allergens in 5 grasses using crossed radioimmunoelectrophoresis (CRIE)]

Using crossed radioimmunoelectrophoresis ( CRIE ) aqueous extracts from pollen of Phleum pratense , Lolium perenne, Poa pratensis , Festuca pratensis and Alopecurus pratensis were investigated for allergen composition. We detected between 24 and 32 antigens. Employing sera from 11 patients with well established hay fever, IgE binding could be demonstrated in 15 out of 28 antigens in Phleum pratense , 13 out of 32 in Lolium perenne, 14 out of 26 in Poa pratensis , 12 out of 24 Festuca pratensis and 12 out of 24 antigens in Alopecurus pratensis . The 11 patients showed an individual pattern of sensitization against the various pollen allergens.