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Seeding Dacron arterial prostheses with peritoneal mesothelial cells: a preliminary morphological study.
The British Journal of Surgery
|July 1, 1984
Summary
Researchers explored using mesothelial cells to line Dacron arterial prostheses in dogs. Seeded grafts showed a significant cellular lining, suggesting a promising alternative to traditional endothelial seeding for vascular grafts.
Area of Science:
- Biomedical Engineering
- Vascular Surgery
- Cell Biology
Background:
- Arterial prostheses are crucial for vascular reconstruction.
- Achieving a functional luminal lining on synthetic grafts remains a challenge.
- Endothelial cell seeding is one approach, but alternatives are being explored.
Purpose of the Study:
- To evaluate the potential of autologous peritoneal mesothelial cells for lining Dacron arterial prostheses.
- To assess the integration and coverage of mesothelial cells on implanted grafts in a canine model.
Main Methods:
- Porous Dacron arterial prostheses were seeded with autologous peritoneal mesothelial cells harvested via collagenase digestion.
- Grafts were implanted into the arterial system of dogs.
- Graft examination using scanning and transmission electron microscopy and morphometric analysis was performed one month post-implantation.
Main Results:
- Control grafts lacked an organized cellular lining.
- Mesothelial cell-seeded grafts demonstrated a cellular lining covering 94% of the luminal surface up to 1 cm from suture lines.
- Mesothelial cells exhibited prostacyclin secretion and fibrinolytic activity.
Conclusions:
- Autologous peritoneal mesothelial cells can form a substantial cellular lining on Dacron arterial prostheses.
- These cells present a potential alternative to endothelium for vascular graft applications.
- The findings support further investigation into mesothelial cell-lined grafts for improved vascular reconstruction.