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Differential activities of rabbit bone marrow suppressor cells
International Archives of Allergy and Applied Immunology
|January 1, 1984
Summary
Nonadherent Fc gamma receptor-bearing (Fc gamma R+) suppressor cells in bone marrow (BM) inhibit constitutive proliferation. However, adherent BM cells suppress immune complex (IC) stimulation, indicating distinct cell populations regulate different cellular processes.
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- Previously identified nonadherent Fc gamma receptor-bearing (Fc gamma R+) suppressor cells in rabbit bone marrow (BM) inhibit constitutive proliferation by blocking a soluble growth factor.
- The interaction between suppressor cells and immune complex (IC) stimulation in BM requires further elucidation.
Purpose of the Study:
- To investigate the differential suppressive activities of Fc gamma R+ and adherent bone marrow cells on constitutive proliferation and lymphocyte activation by immune complexes (IC).
- To explore the role of a soluble growth factor in IC-induced proliferation in the presence or absence of adherent cells.
Main Methods:
- Bone marrow cell cultures from rabbits.
- Assays for constitutive cell proliferation.
- Lymphocyte activation assays using immune complexes (IC).
- Cell adherence and depletion techniques.
Main Results:
- Fc gamma R+ BM suppressor cells inhibited constitutive proliferation but not IC-induced lymphocyte activation.
- Adherent BM cells suppressed IC stimulation but not constitutive proliferation.
- IC-induced proliferation was enhanced by a soluble growth factor only when adherent cells were absent.
- Depletion of Fc gamma R+ cells led to the development of more Fc gamma R+ cells, suggesting increased potential for IC interaction.
Conclusions:
- Distinct bone marrow cell populations differentially regulate constitutive proliferation and immune complex-mediated responses.
- Adherent cells play a crucial role in suppressing immune complex-driven lymphocyte activation.
- The findings suggest a complex interplay between different bone marrow cell subsets in immune regulation and potential interactions with immune complexes.