Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-determined DNA separations.

L S Lerman, S G Fischer, I Hurley

    Annual Review of Biophysics and Bioengineering
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Acceptance of physical activity monitoring in cancer patients during radiotherapy, the GIROfit phase 2 pilot trial.

    Technical innovations & patient support in radiation oncology·2022
    Same author

    Auger-spectroscopy in quantum Hall edge channels and the missing energy problem.

    Nature communications·2019
    Same author

    Radiation Pneumonitis after Intensity-Modulated Radiotherapy for Esophageal Cancer: Institutional Data and a Systematic Review.

    Scientific reports·2019
    Same author

    Systematic Fuel Cavity Asymmetries in Directly Driven Inertial Confinement Fusion Implosions.

    Physical review letters·2017
    Same author

    Duplication events and the evolution of segmental identity.

    Evolution & development·2005
    Same author

    Cloning of the breakpoints of a de novo inversion of chromosome 8, inv (8)(p11.2q23.1) in a patient with Ambras syndrome.

    Cytogenetic and genome research·2004
    Same journal

    Optical sectioning microscopy: cellular architecture in three dimensions.

    Annual review of biophysics and bioengineering·1984
    Same journal

    Magnetic guidance of organisms.

    Annual review of biophysics and bioengineering·1984
    Same journal

    Bacterial motility and the bacterial flagellar motor.

    Annual review of biophysics and bioengineering·1984
    Same journal

    Evolution and the tertiary structure of proteins.

    Annual review of biophysics and bioengineering·1984
    Same journal

    Biophysical applications of quasi-elastic and inelastic neutron scattering.

    Annual review of biophysics and bioengineering·1984
    Same journal

    Immunoelectron microscopy of ribosomes.

    Annual review of biophysics and bioengineering·1984
    See all related articles

    Electrophoretic mobility of DNA reveals sequence-dependent properties, enabling precise separation of DNA molecules. This method allows for the detection and isolation of even single base-pair mutations.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Genetics

    Background:

    • DNA's electrophoretic mobility is influenced by its helix stability and base sequence.
    • Understanding the relationship between DNA helix-random chain transitions and base sequence is crucial for molecular analysis.

    Purpose of the Study:

    • To investigate the link between DNA helix-random chain transitions and base sequence using electrophoretic mobility.
    • To develop a procedure for separating DNA molecules based on their sequence.
    • To demonstrate the predictability of DNA gel behavior for known sequences.

    Main Methods:

    • Utilized statistical mechanical theory to analyze transition equilibria.
    • Incorporated simplified theoretical considerations on strand unravelling's effect on mobility.

    Related Experiment Videos

  • Applied electrophoretic methods to study DNA behavior in gels.

    • Main Results:

    • Demonstrated that DNA gel behavior is predictable for known sequences.
    • Identified cooperative linkage of large base blocks into domains with sharp boundaries.
    • Highlighted the significant role of nearest-neighbor interactions in DNA stability and domain structure.

    Conclusions:

    • Electrophoretic mobility variations under marginal helix stability offer a powerful tool for DNA sequence analysis and separation.
    • This technique enables complete resolution of complex DNA mixtures and detection of single base-pair differences, facilitating mutant sequence isolation.
    • The study reduces the need for extensive sequential analysis by localizing sequence changes within DNA fragments.