Mating call phonotaxis in the female American toad: induction by hormones

Gravid, female American toads are known to move toward conspecific mating calls. This behavior, as well as ovulation, often can be induced by the injection of human chorionic gonadotropin (HCG). It was found that HCG-induced phonotaxis could be terminated by the injection of indomethacin (an inhibitor of prostaglandin synthesis) and then reinstated by the injection of prostaglandin F2 alpha. A drug regimen was devised that allowed elicitation of phonotaxis, indistinguishable from that induced by HCG and in the absence of ovulation. This involved the administration of prostaglandin F2 alpha and arginine vasotocin (or mesotocin) following progesterone priming. It has not been determined whether the peptide is really essential.