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Strain differences among inbred mice in protein kinase C activity
Biochemical and Biophysical Research Communications
|July 31, 1984
Summary
Researchers identified protein kinase C in mouse organs. Its activity varies by organ and mouse strain, with genetic factors influencing levels, suggesting additive inheritance.
Area of Science:
- Biochemistry
- Genetics
- Pharmacology
Background:
- Protein kinase C (PKC) is a family of enzymes crucial for cell signaling.
- Understanding PKC activity and its genetic regulation is important for various biological processes.
Purpose of the Study:
- To characterize protein kinase C activity in different mouse organs.
- To investigate the genetic basis of variations in protein kinase C activity among mouse strains.
Main Methods:
- Assaying protein kinase activity in mouse organ homogenates under specific conditions (Ca++, phosphatidylserine, pH 6).
- Comparing protein kinase C activity levels across seven different mouse strains.
- Analyzing protein kinase C activity in F1 hybrid mice from a cross between A/J and BALB/cByJ strains.
Main Results:
- Protein kinase C activity was detected in mouse brain, spleen, lung, and heart, with highest activity in the brain.
- Seven mouse strains exhibited similar protein kinase C activity levels, except for strain A/J, which showed half the activity in all organs.
- F1 hybrid mice displayed intermediate protein kinase C activity levels, indicating a genetic influence.
Conclusions:
- The identified enzyme exhibits characteristics of protein kinase C.
- Protein kinase C activity shows organ-specific distribution and is subject to genetic control in mice.
- The observed differences in protein kinase C activity among mouse strains are likely due to additive inheritance.