Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

67Ga-binding substances in abscess and normal tissues.

A Ando, I Ando, T Hiraki

    European Journal of Nuclear Medicine
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adverse Drug Event Profile Associated with Anti-dementia Drugs: Analysis of a Spontaneous Reporting Database.

    Die Pharmazie·2023
    Same author

    Weaning stage hyperglycemia induces glucose-insensitivity in arcuate POMC neurons and hyperphagia in type 2 diabetic GK rats.

    Neuropeptides·2018
    Same author

    Inhibition of malic enzyme 1 disrupts cellular metabolism and leads to vulnerability in cancer cells in glucose-restricted conditions.

    Oncogenesis·2017
    Same author

    Rapid Discrimination of Mitochondrial DNA Type and Use of Results to Study Mitochondrial Inheritance in Pleurotus spp.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Synthesis of a Lectin in Both Mycelia and Fruit Bodies of the Ascomycete Mushroom Aleuria aurantia.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Purification and Properties of Amylases Extracellularly Produced by an Imperfect Fungus, Fusidium sp. BX-1 in a Glycerol Medium.

    Bioscience, biotechnology, and biochemistry·2016
    Same journal

    [Not Available].

    European journal of nuclear medicine·2016
    Same journal

    A Window on Latin America.

    European journal of nuclear medicine·2016
    Same journal

    Erratum: Eur J Nucl Med (1999) 26: 686-692.

    European journal of nuclear medicine·2016
    Same journal

    Nuclear medicine in the next decade.

    European journal of nuclear medicine·2014
    Same journal

    Erratum: Impact of attenuation correction on the accuracy of FDG-PET in patients with abdominal tumors: a free-response ROC analysis.

    European journal of nuclear medicine·2014
    Same journal

    (99m)Tc(V)DMSA quantitatively predicts (188)Re(V)DMSA distribution in patients with prostate cancer metastatic to bone.

    European journal of nuclear medicine·2014
    See all related articles

    Gallium-67 (67Ga) citrate preferentially binds to sulfated acid mucopolysaccharides in abscesses and major organs. These findings identify key binding substances for 67Ga in both healthy and infected tissues.

    Area of Science:

    • Biomedical imaging
    • Radiopharmaceuticals
    • Biochemistry

    Background:

    • Gallium-67 citrate (67Ga) is a radiopharmaceutical used in diagnostic imaging.
    • The precise binding mechanisms and targets of 67Ga in various tissues, particularly in the presence of abscesses, require further elucidation.

    Purpose of the Study:

    • To identify the specific substances in abscesses and normal organs that bind to 67Ga.
    • To investigate the molecular characteristics of these 67Ga-binding substances.

    Main Methods:

    • Animals with induced abscesses and normal animals were administered 67Ga-citrate.
    • Tissues (abscess, kidney, heart, lung, spleen) were homogenized and fractionated.
    • Supernatants were analyzed using Sephadex G-100 chromatography.

    Related Experiment Videos

  • Eluates were assessed for radioactivity and biochemical components, including protein, uronic acids, and sialic acids.
  • Sodium sulfate-35S was used to identify sulfated compounds.

    • Main Results:

    • Significant amounts of 67Ga were found bound to sulfated acid mucopolysaccharides in abscesses, kidneys, lungs, hearts, and spleens.
    • Two distinct molecular weight fractions of sulfated acid mucopolysaccharides (approx. 10,000 and >40,000 Da) showed 67Ga binding.
    • The binding substances were confirmed to be sulfated acid mucopolysaccharides.

    Conclusions:

    • Sulfated acid mucopolysaccharides are the primary 67Ga-binding substances in abscesses.
    • These mucopolysaccharides are also the main targets for 67Ga in healthy kidney, heart, lung, and spleen tissues.
    • This study clarifies the molecular basis of 67Ga localization in infected and normal tissues.