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Nicotinamide methylation. Tissue distribution, developmental and neoplastic changes.

R Seifert, J Hoshino, H Kröger

    Biochimica Et Biophysica Acta
    |September 28, 1984
    PubMed
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    Nicotinamide methylase activity is highest in adult rat liver and significantly decreases in most tumors. Enzyme levels change during development, with lower activity in logarithmically growing cultured cells.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Cancer Research

    Background:

    • Nicotinamide:S-adenosylmethionine methyltransferase (nicotinamide methylase) is crucial for methylation reactions.
    • Understanding its distribution and regulation is important for cellular processes and disease states.

    Purpose of the Study:

    • To investigate the distribution of nicotinamide methylase activity in normal adult rat and mouse tissues.
    • To examine changes in enzyme activity during rat tissue development.
    • To analyze nicotinamide methylase activity in various tumors and cultured cells.

    Main Methods:

    • Enzyme activity assays were performed on tissue homogenates and cultured cells.
    • Comparative analysis of enzyme activity across different tissues, developmental stages, and tumor types.

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  • Investigation of enzyme activity in relation to cell growth phases.

    • Main Results:

    • Adult rat liver showed the highest nicotinamide methylase activity; other tissues had less than 15% of this level.
    • Fetal liver exhibited low activity, increasing to adult levels by postnatal day 28.
    • Most hepatomas and tumors showed decreased activity, inversely correlated with growth rate; cultured cells in stationary phase had higher activity than proliferating cells.

    Conclusions:

    • Nicotinamide methylase activity varies significantly across normal tissues and developmental stages.
    • Reduced nicotinamide methylase activity is a common feature in many tumors, potentially linked to malignancy.
    • Cell cycle and growth phase influence nicotinamide methylase activity in cultured liver cells.