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Related Experiment Videos

Ehrlich ascites cells activate the alternative pathway of the human complement system.

M Betz, G M Hänsch, M L Shin

    Immunobiology
    |May 1, 1984
    PubMed
    Summary

    The alternative complement pathway, a key immune response, effectively kills Ehrlich ascites cells. This cell death occurs independently of calcium ions, indicating a robust activation mechanism.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Complement System

    Background:

    • The complement system is a crucial part of innate immunity.
    • Complement activation can proceed through classical, lectin, or alternative pathways.
    • Ehrlich ascites cells are a model for studying cell-mediated immune responses.

    Purpose of the Study:

    • To investigate the mechanism of Ehrlich ascites cell killing by human sera.
    • To determine the role of the complement system, specifically the alternative pathway, in this process.

    Main Methods:

    • Incubation of Ehrlich ascites cells with normal, C1q-deficient, and C2-deficient human sera.
    • Assessment of cell viability.
    • Analysis of complement factor cleavage (Factor B and C3).

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  • Experiments conducted with and without free calcium ions.

    • Main Results:

    • Ehrlich ascites cells were killed by both normal and complement-deficient sera.
    • Cell killing occurred even in the absence of free calcium ions.
    • Cleavage of Factor B and C3 was observed, indicating complement activation.

    Conclusions:

    • The alternative pathway of the complement system is activated on the surface of Ehrlich ascites cells.
    • Complement-mediated killing of these cells is calcium-independent.
    • This suggests a significant role for the alternative pathway in targeting tumor cells.