Isolation and characterization of surface glycoproteins from L-1210, P-388 and HeLa cells

A method is described that permits the rapid extraction of the cell surface glycoproteins of two murine leukemic cells, the P-388 and the L-1210 cells as well as those of the human adenocarcinoma cells, the HeLa cells. Proof of the surface location of these glycoproteins is provided by labeling the intact cells; (a) with 125I by the lactoperoxidase iodination technique; (b) with 3H by the galactose oxidase-reductive tritiation method. Most of these glycoproteins were also shown to incorporate radioactive glucosamine and fucose. By these criteria as well as by the distribution of molecular weights, the surface glycoproteins of the two murine cells are indistinguishable; however, they differ markedly from the surface glycoproteins of HeLa cells. The extracts of the murine cells were shown to contain lectin receptor activity as determined by their ability to inhibit the lectin-induced agglutination of the intact cells.