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Diamine oxidase activity in human decidua and endometrium
American Journal of Obstetrics and Gynecology
|October 15, 1984
Summary
Diamine oxidase activity significantly increases in human decidua during early pregnancy, indicating its synthesis and secretion. Serum factors, not hormones, appear to stimulate this enzyme
Area of Science:
- Reproductive biology
- Enzymology
- Biochemistry
Background:
- Diamine oxidase (DAO) activity elevates significantly in pregnant women's blood.
- Progestins are known to stimulate DAO in rodent uteri.
- DAO regulates cell proliferation and immune responses.
Purpose of the Study:
- To investigate DAO activity in human endometrium across the menstrual cycle and in early pregnancy decidua.
- To assess the influence of estradiol and medroxyprogesterone acetate on DAO activity in cultured human tissues.
- To characterize DAO kinetics and substrate interactions.
Main Methods:
- Assay of DAO activity in human endometrial and decidual tissues.
- Organ culture of endometrium and decidua with subsequent media analysis.
- Enzyme kinetic studies using putrescine as substrate and histamine/spermidine as inhibitors.
- Hormonal treatment of cultured tissues with estradiol and medroxyprogesterone acetate.
Main Results:
- First-trimester decidua exhibited significantly higher DAO activity than endometrium.
- DAO activity increased in decidua from 6 to 17 weeks of gestation.
- Decidua actively secreted DAO into culture media, with activity enhanced by bovine serum but not by tested hormones.
- Kinetic analysis revealed specific substrate affinity and competitive inhibition by histamine and spermidine.
Conclusions:
- Human decidua synthesizes and exports significant amounts of diamine oxidase.
- Serum factors, rather than estradiol or medroxyprogesterone acetate, appear to modulate DAO activity in early pregnancy.
- DAO plays a role in early pregnancy, with specific substrate interactions.