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Monocyte/T-cell interactions in Con A induced suppressor activity.

B Davidsen, E Kristensen

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology

|June 1, 1984

Summary

Monocytes are not required for concanavalin-A (Con A) induced suppressor activity in peripheral blood mononuclear cells (PBMC). These cells do not contribute to or mediate Con A-driven suppression in lymphocyte activation.

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Area of Science:

Immunology
Cellular Biology

Background:

Peripheral blood mononuclear cells (PBMC) are known to exhibit concanavalin-A (Con A) induced suppressor activity.
The specific role of monocytes within PBMC populations in mediating this suppressor activity has been a subject of investigation.

Purpose of the Study:

To investigate the necessity of monocytes for concanavalin-A (Con A) induction of suppressor activity in peripheral blood mononuclear cells (PBMC).
To determine if monocytes themselves possess any Con A-dependent suppressor potential.
To assess the role of monocytes in enabling Con A-activated lymphocytes to exert suppressor activity.

Main Methods:

Examination of peripheral blood mononuclear cell (PBMC) populations with varying monocyte content.
Assessment of concanavalin-A (Con A) induced suppressor activity.
Evaluation of Con A-stimulated lymphoblast-transformation assays.

Main Results:

Monocytes were found to be unnecessary for the concanavalin-A (Con A) induction of suppressor activity in PBMC.
Monocytes did not exhibit any inherent Con A-dependent suppressor potential.
The presence of monocytes was not required for Con A-activated lymphocytes to suppress responder systems, such as Con A-stimulated lymphoblast-transformation.

Conclusions:

Monocytes are not essential components for the concanavalin-A (Con A) mediated suppression observed in peripheral blood mononuclear cells (PBMC).
The suppressor activity induced by Con A in PBMC populations is primarily mediated by non-monocyte cell types.
These findings clarify the cellular basis of Con A-induced immune suppression, highlighting the role of lymphocytes independent of monocytes.

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