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Circulating Sézary cells. A new preparatory method for their identification and enumeration
Archives of Pathology & Laboratory Medicine
|December 1, 1984
Summary
Enumerating Sézary cells, abnormal lymphoid cells, is crucial for diagnosing mycosis fungoides and Sézary syndrome. A new method using density gradients and plastic embedding improves identification and counting of these cells.
Area of Science:
- Dermatology
- Hematology
- Cell Biology
Background:
- Sézary cells are abnormal lymphoid cells important in diagnosing Sézary syndrome and mycosis fungoides.
- Current methods for enumerating Sézary cells lack widespread acceptance due to practicality and accuracy issues.
Purpose of the Study:
- To describe a novel, practical, and accurate method for identifying and enumerating circulating Sézary cells.
Main Methods:
- Peripheral blood mononuclear cells were isolated using density gradient separation.
- Cells were embedded in plastic, sectioned, and stained for alpha-naphthyl acetate esterase.
- Identification and enumeration were performed using light microscopy.
Main Results:
- The developed method allows for effective identification and enumeration of Sézary cells.
- The percentage of Sézary cells positively correlated with the clinical diagnosis of Sézary syndrome.
Conclusions:
- This new technique offers a practical and accurate approach for Sézary cell enumeration.
- Accurate enumeration aids in the evaluation of mycosis fungoides, Sézary syndrome, and related dermatoses.