Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subunit interactions in human plasma fibronectin.

R M Robinson, J Hermans

    Biochemical and Biophysical Research Communications
    |November 14, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Gastric lymphomas compared with lymph node lymphomas in a population-based registry differ in stage distribution and dissemination patterns but not in patient survival.

    Cancer·1997
    Same author

    Stepwise compared with rapid application of vacuum in ventouse extraction procedures.

    British journal of obstetrics and gynaecology·1997
    Same author

    Two autonomous nodules of a patient with multinodular goiter harbor different activating mutations of the thyrotropin receptor gene.

    The Journal of clinical endocrinology and metabolism·1997
    Same author

    Accuracy of the Jamshidi trocar biopsy in the diagnosis of bone tumors.

    Clinical orthopaedics and related research·1997
    Same author

    Allogeneic bone marrow transplantation versus autologous stem cell transplantation in multiple myeloma: a retrospective case-matched study from the European Group for Blood and Marrow Transplantation.

    Blood·1996
    Same author

    Splenic irradiation before bone marrow transplantation for chronic myeloid leukaemia. Chronic Leukaemia Working Party of the European Group for Blood and Marrow Transplantation (EBMT)

    British journal of haematology·1996
    Same journal

    NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

    Biochemical and biophysical research communications·2026
    Same journal

    Deciphering potent MPL activation by the fucose-binding lectin thrombocorticin.

    Biochemical and biophysical research communications·2026
    Same journal

    Mitochondrial genome alterations in cancer: From mutations and epigenetics to targeted therapiesack.

    Biochemical and biophysical research communications·2026
    Same journal

    GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

    Biochemical and biophysical research communications·2026
    Same journal

    A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

    Biochemical and biophysical research communications·2026
    Same journal

    Development of a vitamin-related gene signature to predict the immune characteristics and prognosis of glioma.

    Biochemical and biophysical research communications·2026
    See all related articles

    Mild reduction splits fibronectin into two chains, but its compact structure persists. Specific noncovalent contacts between fibronectin chains stabilize its native conformation.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • Fibronectin is a large extracellular matrix glycoprotein with a complex molecular structure.
    • Understanding the forces stabilizing fibronectin's conformation is crucial for comprehending its biological functions.

    Purpose of the Study:

    • To investigate the structural integrity of fibronectin after chemical reduction.
    • To determine the role of inter-chain interactions in maintaining fibronectin's compact native state.

    Main Methods:

    • Chemical reduction of fibronectin using dithiothreitol.
    • Analysis of physical properties including light scattering and gel exclusion chromatography.
    • Assessment of fibronectin conformation in the presence and absence of denaturants and glycerol.

    Related Experiment Videos

    Main Results:

    • Mild reduction cleaved fibronectin into two chains (mol. wt. 220,000) without altering physical properties.
    • Physical properties remained consistent with intact fibronectin, except in denaturants, which induced an open conformation.
    • Plasmin digestion to fragments (mol. wt. < 200,000) halved light scattering intensity in 30% glycerol but not without it.

    Conclusions:

    • Fibronectin's compact native conformation is stabilized by specific noncovalent contacts between its constituent chains.
    • These inter-chain interactions are essential for maintaining fibronectin's structural integrity and function.