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Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
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Aortic pressure, substrate utilization and protein synthesis.

H E Morgan, Y Kira, E E Gordon

    European Heart Journal
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Increased aortic pressure in Langendorff perfused hearts boosts heart metabolism and protein synthesis. Ventricular wall stretch, not just pressure, appears to drive these metabolic changes.

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    Area of Science:

    • Cardiovascular Physiology
    • Cardiac Metabolism
    • Biomechanical Forces in the Heart

    Background:

    • Aortic pressure influences cardiac workload and metabolism.
    • The precise mechanisms linking pressure changes to metabolic activity require further elucidation.

    Purpose of the Study:

    • To investigate the impact of elevated aortic pressure on myocardial metabolic functions.
    • To differentiate the roles of pressure load versus mechanical stretch in mediating these effects.

    Main Methods:

    • Utilizing Langendorff perfused hearts subjected to varying aortic pressures.
    • Employing ventricular drainage to isolate pressure effects from developed intraventricular pressure.
    • Assessing oxygen consumption, substrate utilization, and protein synthesis rates.
    • Measuring energy availability via creatine-phosphate to creatine ratios.

    Main Results:

    • Elevated aortic pressure (60-120 mmHg) increased oxygen consumption, glucose utilization, pyruvate oxidation, and protein synthesis.
    • These metabolic increases persisted even when intraventricular pressure development was prevented.
    • Cardiac arrest abolished the pressure-induced increase in oxygen consumption.
    • Protein synthesis increased with aortic pressure elevation in arrested hearts, irrespective of substrate (glucose or pyruvate).
    • Energy availability increased with aortic pressure in glucose-perfused hearts but not pyruvate-perfused hearts.

    Conclusions:

    • Ventricular wall stretch, induced by increased aortic pressure, is a key mechanical factor driving metabolic adaptations in the heart.
    • These findings highlight the role of mechanical stimuli in regulating cardiac energy metabolism and synthesis processes.