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Related Experiment Videos

A standardized technique for efficient platelet and leukocyte collection using the Model 30 Blood Processor.

J Aisner, C A Schiffer, J H Wolff

    Transfusion
    |September 1, 1976
    PubMed
    Summary

    The Model 30 Blood Processor efficiently separates blood components like platelets and lymphocytes. Optimizing collection techniques with specific anticoagulants, such as hydroxyethyl starch for granulocytes, enhances yield and minimizes red blood cell loss.

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    Area of Science:

    • Hematology
    • Biomedical Engineering
    • Cellular Biology

    Background:

    • Current blood cell component harvesting relies on subjective visual assessment.
    • Objective methods are needed to optimize the efficiency and yield of blood cell separation.

    Purpose of the Study:

    • To evaluate the efficiency of the Model 30 Blood Processor for harvesting specific blood cell components.
    • To determine optimal anticoagulant and collection strategies for maximizing cell yield.

    Main Methods:

    • Serial sampling and analysis of blood fractions from the Model 30 Blood Processor.
    • Testing of acid-citrate-dextrose (ACD), heparin, and hydroxyethyl starch (HES) as anticoagulants.
    • Graphed and tabulated data to assess cell component overlap and harvest efficiency.

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    Main Results:

    • High yields of lymphocytes (approx. 80%) with ACD or heparin.
    • Efficient platelet harvesting (approx. 90%) using ACD.
    • Improved granulocyte harvesting (approx. 70%) with HES by extending collection into the red blood cell layer.
    • ACD resulted in poor granulocyte recovery (<10%).

    Conclusions:

    • The Model 30 Blood Processor is highly efficient for blood cell separation.
    • Anticoagulant choice significantly impacts the yield of specific cell types.
    • Developed a standardized technique for optimized cell collection, though intermittent flow limits processing volume.