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Related Experiment Videos

Met-enkephalin circulates in human plasma.

V Clement-Jones, P J Lowry, L H Rees

    Nature
    |January 17, 1980
    PubMed
    Summary

    Met-enkephalin, a key neurotransmitter, circulates in human plasma and may be secreted by the adrenal gland. This study confirms its presence as an intact pentapeptide, clarifying its physiological roles.

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    Area of Science:

    • Neuroscience
    • Endocrinology
    • Biochemistry

    Background:

    • The physiological roles of Met-enkephalin and Leu-enkephalin remain largely unknown.
    • These peptides are suspected neurotransmitters in both central and peripheral nervous systems.
    • Previous detection methods for Met-enkephalin showed cross-reactivity, casting doubt on its presence in human tissues.

    Purpose of the Study:

    • To definitively establish the presence and form of Met-enkephalin in human plasma.
    • To investigate the potential secretion of Met-enkephalin by the adrenal gland.

    Main Methods:

    • Utilized a highly specific radioimmunoassay (RIA) for Met-enkephalin detection.
    • Employed chromatographic analysis to determine the molecular form of Met-enkephalin in plasma.

    Main Results:

    • Evidence obtained for Met-enkephalin-like material circulating in the plasma of normal subjects.
    • The adrenal gland is suggested as a potential source of plasma Met-enkephalin.
    • Chromatographic analysis revealed plasma Met-enkephalin exists primarily as the intact pentapeptide.

    Conclusions:

    • The study provides strong evidence for the existence of Met-enkephalin in human plasma.
    • Findings suggest Met-enkephalin circulates as the active pentapeptide, not a degraded form.
    • This research clarifies previous ambiguities regarding Met-enkephalin's presence and form in humans.

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