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Studies of renal cell function using cell culture techniques.

J S Handler, F M Perkins, J P Johnson

    The American Journal of Physiology
    |January 1, 1980
    PubMed
    Summary
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    Cell culture models, including kidney and toad bladder cells, enable detailed study of renal cell function, transport mechanisms, and hormonal responses. These models provide insights into cellular processes and disease mechanisms.

    Area of Science:

    • Cell Biology
    • Renal Physiology
    • Biochemistry

    Background:

    • Cell culture provides a versatile platform for investigating complex cellular functions.
    • Epithelial cells in culture mimic in vivo structures and functions, facilitating research on renal cell biology.
    • Established cell lines offer reproducible models for studying specific physiological processes.

    Purpose of the Study:

    • To explore the utility of cell culture in studying renal cell function and epithelial biology.
    • To investigate transepithelial transport, hormonal regulation, and molecular mechanisms in kidney and bladder epithelial cells.
    • To examine the biosynthesis of vitamin D metabolites and prostaglandin production in cultured renal cells.

    Main Methods:

    • Utilizing established mammalian kidney cell lines (MDCK, LLC-PK1) and toad urinary bladder cell lines.

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  • Assessing transepithelial salt and water transport, glucose transport, and adenylate cyclase activity.
  • Investigating responses to hormones (e.g., aldosterone) and drugs (e.g., amiloride), including cyclic AMP modulation.

    • Main Results:

    • Demonstrated transepithelial transport of salt and water in mammalian kidney cell lines.
    • Observed glucose transport systems and hormone-sensitive adenylate cyclase activity in kidney cell lines.
    • Characterized sodium transport, its stimulation by cyclic AMP and aldosterone, and differential amiloride sensitivity in toad bladder cell lines.

    Conclusions:

    • Cell cultures of kidney and toad bladder epithelia are valuable models for studying renal physiology and transport.
    • Investigating differences in cellular responses to aldosterone and amiloride can elucidate molecular mechanisms.
    • Cell culture facilitates research on vitamin D metabolism, prostaglandin synthesis, and kidney developmental biology.