Improvement of enterovirus neutralization by treatment with sodium deoxycholate or chloroform

Enterovirus strains which could be neutralized only partially or not at all by specific antiserum were readily accessible to the antibodies after treatment with sodium deoxycholate or chloroform. The latter treatment is simple and can be performed with crude virus suspensions of low titer. The method was particularly successful for routine typing of enterovirus 71 and coxsackievirus types A7 and A16.