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Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses
Abstract:
Recombinant mink cell focus-inducing (MCF) murine leukemic viruses, as well as ecotropic and xenotropic viruses, were tested for ability to accelerate or cause development of lymphoma in AKR and other strains of mice. Of the three classes of virus isolated from AKR, only the MCF viruses were able to accelerate development of AKR lymphoma. This fully supports the idea that the MCF viruses are the proximal cause of spontaneous AKR lymphoma. MCF lymphomagenicity was strain specific, however, in that AKR MCF viruses did not induce lymphomas in many murine strains; they were moderately lymphomagenic in C3H/Bi mice and in National Institutes of Health Swiss partially congenic for Akv-1 or Akv-2. In contrast, MCF viruses from nonthymic hematopoietic neoplasms of C3H/Fg, BALB/c, or mice partially congenic for ecotropic virus loci (Akv-1, Akv-2, Fgv-1, C58v-1, and C58v-2) were not able to accelerate or cause lymphomia in AKR or any other mouse strain tested, including some of the strains of origin. MCF lymphomagenicity correlated with thymic origin in the virus and with ability to replicate in the thymus.
Insights
Mink cell focus-inducing (MCF) murine leukemic viruses accelerate lymphoma in AKR mice, confirming their role in spontaneous lymphoma. However, this lymphomagenicity is strain-specific and linked to thymic origin and replication.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Murine leukemia viruses are implicated in lymphoma development.
- Different virus classes, including mink cell focus-inducing (MCF), ecotropic, and xenotropic viruses, exist.
- Spontaneous lymphoma in AKR mice is a well-studied model.
Purpose of the Study:
- To investigate the role of different murine leukemia virus classes in lymphoma development.
- To determine if MCF viruses are the proximal cause of spontaneous AKR lymphoma.
- To assess the strain specificity and biological correlates of MCF lymphomagenicity.
Main Methods:
- Testing recombinant MCF, ecotropic, and xenotropic murine leukemic viruses.
- Inoculating various mouse strains, including AKR, C3H/Bi, and NIH Swiss congenic mice.
- Analyzing virus origin (thymic vs. nonthymic) and replication capacity in the thymus.
Main Results:
- MCF viruses isolated from AKR mice accelerated lymphoma development in AKR mice.
- AKR MCF viruses showed strain-specific lymphomagenicity, with moderate effects in some strains.
- MCF viruses from nonthymic sources or other strains did not induce lymphoma in AKR or other tested mice.
- Lymphomagenicity correlated with thymic origin and replication in the thymus.
Conclusions:
- MCF viruses are the proximal cause of spontaneous AKR lymphoma.
- MCF lymphomagenicity is dependent on virus origin and host strain.
- Thymic origin and replication are key factors in MCF virus-induced lymphomagenesis.