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Enzyme-linked immunosorbent assay for detection of poliovirus antigen and antibody
Developments in Biological Standardization
|January 1, 1980
Abstract:
A sensitive method is described to detect poliovirus antigen in diluted samples of semi-processed and processed Salk vaccine material using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The introduction of a simple conjugate-blocking step permits confirmation of antigen specificity and allows evaluation of neutralising antibody concentration in the sera employed as soon as four hours after sample addition.