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Concentration and purification of poliovirus by immune adsorption on immobilized antibodies
Antibodies to poliovirus type 1, 2 and 3 have been coupled to Sepharose 4 B. The immune sorbentia have high binding capacities for both live and inactivated virus. Bound antigen is eluted with a high concentration of ammoniumisothiocyanate. Immediately after elution the chaotropic salt is removed by gelfiltration. The antigen recovery with inactivated virus was low; however, with live virus a good recovery was obtained. Therefore, immune adsorption seems to be applicable for concentration and purification of large amounts of live poliovirus.
Antibodies to poliovirus type 1, 2 and 3 have been coupled to Sepharose 4 B. The immune sorbentia have high binding capacities for both live and inactivated virus. Bound antigen is eluted with a high concentration of ammoniumisothiocyanate. Immediately after elution the chaotropic salt is removed by gelfiltration. The antigen recovery with inactivated virus was low; however, with live virus a good recovery was obtained. Therefore, immune adsorption seems to be applicable for concentration and purification of large amounts of live poliovirus.