Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Use of plasma membrane vesicles.

G Sachs, R J Jackson, E C Rabon

    The American Journal of Physiology
    |March 1, 1980
    PubMed
    Summary

    This study reviews methods for obtaining and characterizing epithelial plasma membrane vesicles. It highlights techniques for assessing vesicle properties and quantitating transport events, crucial for protein studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Associations between disturbed sleep and attenuated psychotic experiences in people at clinical high risk for psychosis.

    Psychological medicine·2024
    Same author

    Editorial: control of acid secretion.

    Alimentary pharmacology & therapeutics·2018
    Same author

    Letter: vonoprazan, a long-lasting acid suppressor of the gastric H+, K+-ATPases with - implications for renal H+, K+-ATPases; authors' reply.

    Alimentary pharmacology & therapeutics·2016
    Same author

    Corrigendum. The binding selectivity of vonoprazan (TAK-438) to the gastric H+,K+-ATPase.

    Alimentary pharmacology & therapeutics·2016
    Same author

    The binding selectivity of vonoprazan (TAK-438) to the gastric H+, K+ -ATPase.

    Alimentary pharmacology & therapeutics·2015
    Same author

    Colloidal bismuth subcitrate impedes proton entry into Helicobacter pylori and increases the efficacy of growth-dependent antibiotics.

    Alimentary pharmacology & therapeutics·2015

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Epithelial plasma membrane vesicles are vital for studying transport mechanisms.
    • Characterizing these vesicles is essential for accurate biological research.

    Purpose of the Study:

    • To summarize current methodologies for obtaining and characterizing epithelial plasma membrane vesicles.
    • To emphasize techniques for evaluating vesicle homogeneity, tightness, orientation, and activity.
    • To discuss methods for quantitating transport events and electrical phenomena.

    Main Methods:

    • Review of vesicle isolation and characterization techniques.
    • Application of isotopic, osmotic, and probe assays for quantitation.
    • Discussion of challenges in transport protein purification and reconstitution.

    Main Results:

    • Detailed overview of vesicle assessment parameters.
    • Illustration of quantitative techniques for transport studies.
    • Identification of key challenges in protein purification and reconstitution.

    Conclusions:

    • Effective characterization of epithelial plasma membrane vesicles is achievable through various methods.
    • Quantitative techniques are crucial for understanding transport events.
    • Further research is needed to overcome challenges in transport protein purification and reconstitution.

    Related Experiment Videos