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Related Experiment Videos

Evidence for fixed charge in the nexus.

P R Brink, M M Dewey

    Nature
    |May 8, 1980
    PubMed
    Summary

    Gap junctions facilitate cell communication via aqueous channels. This study suggests these channels possess fixed anionic charges, influencing molecule permeability and potentially binding amino groups.

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    Area of Science:

    • Cell Biology
    • Neuroscience
    • Biophysics

    Background:

    • Gap junctions (nexuses) are low-resistance, permeable junctions facilitating intercellular communication.
    • Previous research suggested nexal channels might have fixed charges or smaller diameters.

    Purpose of the Study:

    • To investigate the structural properties of gap junction channels.
    • To determine if fixed charges exist within the nexal channel of Lumbricus terrestris median giant axon.

    Main Methods:

    • Examined the permeability of nexuses using various fluorescein derivatives.
    • Utilized Lumbricus terrestris median giant axon septa for experiments.
    • Compared observed fluorescein permeability with predictions based on molecular size and weight.

    Main Results:

    • Carboxyfluorescein and aminofluorescein showed depressed permeability.
    • Aminofluorescein pre-injection significantly suppressed fluorescein diffusion.
    • Carboxyfluorescein pre-injection did not affect fluorescein diffusion.

    Conclusions:

    • Nexal channels in Lumbricus terrestris likely contain fixed anionic charges.
    • These anionic charges may exhibit affinity for amino groups, influencing permeability.
    • Findings contribute to understanding gap junction channel properties and regulation.

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