Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The lambda phage att site: functional limits and interaction with Int protein.

P L Hsu, W Ross, A Landy

    Nature
    |May 8, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The use of methylprednisolone after third molar surgery. A systematic review and meta-analysis of randomized controlled trials.

    Medicina oral, patologia oral y cirugia bucal·2024
    Same author

    Incidence of Parkinson disease in North America.

    NPJ Parkinson's disease·2022
    Same author

    Injuries in Slow-Pitch Softball.

    The Physician and sportsmedicine·2016
    Same author

    Effect of guava juice and papaya on the acidity of the urine.

    Journal of the American Dietetic Association·2010
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    The B vitamins of pork tissues from pigs fed garbage and grain rations.

    Proceedings of the annual meeting. Hawaiian Academy of Science·2010
    Same journal

    Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

    Nature·2026
    Same journal

    China boosts prestigious grants for young scientists - will it ease competition?

    Nature·2026
    Same journal

    Incoming US science academy chief vows to 'double down' on research.

    Nature·2026
    Same journal

    Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

    Nature·2026
    Same journal

    Electrodeposited self-assembled molecules for perovskite photovoltaics.

    Nature·2026
    Same journal

    Neutrino's nursery found: the 'Shadow Blaster'.

    Nature·2026
    See all related articles

    Bacteriophage lambda recombination uses unequal att sites. The larger donor site, with crucial Int protein binding, efficiently integrates with a smaller recipient site lacking these interactions.

    Area of Science:

    • Molecular biology
    • Genetics
    • Virology

    Background:

    • Site-specific recombination is a key mechanism in viral genome integration.
    • Bacteriophage lambda utilizes Int protein for integration into host DNA.

    Purpose of the Study:

    • To elucidate the structural and functional differences between the donor and recipient att sites in bacteriophage lambda recombination.
    • To understand the role of Int protein binding sites in mediating recombination efficiency.

    Main Methods:

    • Analysis of the bacteriophage lambda att site structure.
    • Investigating the binding of Int protein to different att site components.

    Main Results:

    • The minimal att site is approximately 240 base pairs.

    Related Experiment Videos

  • Four Int protein binding sites exist within the att site, with at least three essential for function.
  • The donor site, rich in Int protein interactions, recombines more efficiently than the smaller recipient site.

    • Conclusions:

    • Recombination efficiency in bacteriophage lambda is dependent on the asymmetric nature of the att sites.
    • Specific Int protein binding sites on the donor att site are critical for efficient integration.