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Human T cell response to Herpes simplex virus antigen in vitro
Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
|February 1, 1980
Summary
Herpes simplex virus type I antigen (HSV-Ag) triggers a T cell proliferative response in individuals with recurrent herpes labialis. This immune reaction requires macrophages and is influenced by T cell donor HLA-D/DR types.
Area of Science:
- Immunology
- Virology
- Cellular Biology
Background:
- Recurrent herpes labialis is often caused by Herpes simplex virus type I (HSV-I).
- T cell responses are crucial for adaptive immunity against viral infections.
- Understanding HSV-I specific T cell responses can inform therapeutic strategies.
Purpose of the Study:
- To investigate the in vitro proliferative response of T cells to HSV-I antigen (HSV-Ag) in individuals with a history of recurrent herpes labialis.
- To determine the requirements for this T cell response, including the role of macrophages and presensitization.
- To explore the influence of human leukocyte antigen (HLA) class II determinants on the HSV-Ag specific T cell response.
Main Methods:
- Isolation of T cells and adherent cells (macrophages) from donors with a history of recurrent herpes labialis.
- In vitro stimulation of T cells with HSV-Ag in the presence or absence of adherent cells.
- Quantification of T cell proliferation.
- Analysis of T cell response in relation to the ratio of T cells to adherent cells.
- Preliminary investigation of HLA-D/DR restriction.
Main Results:
- HSV-Ag induced a significant T cell proliferative response in vitro.
- The response was dependent on the presence of presensitized T cells and adherent cells (macrophages).
- Optimal T cell proliferation occurred at a 10:1 ratio of T cells to adherent cells.
- Preliminary data suggested HLA-D/DR restriction of the HSV-Ag specific T cell response.
Conclusions:
- T cells from individuals with recurrent herpes labialis can mount an in vitro proliferative response to HSV-Ag.
- This response necessitates both presensitization and the presence of macrophages.
- The magnitude of the response is influenced by the number of adherent cells.
- The HSV-Ag specific T cell response appears to be restricted by HLA-D/DR determinants, indicating a role for antigen-presenting cells in T cell activation.