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Updated: Aug 13, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
Paramagnetic isoprenoid carrier lipids. 2. Dispersion and dynamics in lipid membranes
Abstract:
The transbilayer diffusion rates and self-association of spin-labeled glycosyl carrier lipids and other isoprenoids have been studied in model phospholipid membranes. Transposition rates of phosphorylated species in small (300 A) or large (greater than or equal to 1000 A) diameter unilamellar phosphatidylcholine (PC) vesicles are slow (t1/2 greater than 5 h at 25 degrees C); this argues against their proposed role in the transbilayer portage of sugar units during polysaccharide and glycoprotein assembly. The same probes mix well with several host lipids, remaining monomolecularly dispersed even at high ionic strength (0.5 M NaCl) or in the presence of various polyvalent cations. This behavior persists for several degrees beneath the transition temperature (Tc) of saturated lecithins. In contrast, neutral carboxylate analogues undergo pronounced reversible self-association. Aggregation is quite dependent on the probe concentration and nature of the host but only weakly temperature dependent above the Tc. Even at low probe concentrations (less than 0.5 mol %) in fluid membranes, aggregates persist above 75 degrees C. Unsaturation in the lecithin fatty acyl chains dramatically increases isoprenoid monomer solubility. Segregation appears to involve relocation of the entire molecule in the membrane interior.
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