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Ultraviolet light--denatured DNA/anti-ultraviolet light--denatured DNA immune-complex nephritis in rabbits
Two groups of preimmunized rabbits were studied during a 3-month course of daily intravenous injections of uv DNA in amounts sufficient to neutralize circulating antibody. One group was given high-molecular-weight uv DNA, and the other group, US uv DNA. Rabbits receiving US uv DNA formed potentially more damaging immune complexes, since this group of animals developed greater rises in blood urea and greater falls in C3. Circulating immune complexes were demonstrated in both groups of animals with the macromolecular IgG assay. Both groups of animals developed evidence of immune complex-mediated glomerular nephritis as evidenced by heavy granular deposits of IgG and C3 in the glomeruli. The results suggest that immune complexes formed with US uv DNA may be more nephrotoxic. This study provides further direct evidence that DNA/anti-DNA immune complexes are capable of glomerular localization with resulting tissue damage.
Two groups of preimmunized rabbits were studied during a 3-month course of daily intravenous injections of uv DNA in amounts sufficient to neutralize circulating antibody. One group was given high-molecular-weight uv DNA, and the other group, US uv DNA. Rabbits receiving US uv DNA formed potentially more damaging immune complexes, since this group of animals developed greater rises in blood urea and greater falls in C3. Circulating immune complexes were demonstrated in both groups of animals with the macromolecular IgG assay. Both groups of animals developed evidence of immune complex-mediated glomerular nephritis as evidenced by heavy granular deposits of IgG and C3 in the glomeruli. The results suggest that immune complexes formed with US uv DNA may be more nephrotoxic. This study provides further direct evidence that DNA/anti-DNA immune complexes are capable of glomerular localization with resulting tissue damage.