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Related Experiment Videos

Collagenase: an experimental study of intervertebral disc dissolution.

J W Bromley, J W Hirst, M Osman

    Spine
    |March 1, 1980
    PubMed
    Summary

    This study shows purified collagenase (Nucleolysin) effectively dissolves nucleus pulposus in animal models. The enzyme demonstrated safety for local tissue, with a suggested safety margin for intrathecal use in monkeys.

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    Area of Science:

    • Biochemistry
    • Veterinary Medicine
    • Enzymology

    Background:

    • The nucleus pulposus is a key component of intervertebral discs.
    • Degenerative disc disease necessitates novel therapeutic approaches.
    • Enzymatic dissolution offers a potential minimally invasive treatment strategy.

    Purpose of the Study:

    • To evaluate the efficacy of purified collagenase (Nucleolysin) for nucleus pulposus dissolution.
    • To assess the local and systemic toxicity of Nucleolysin.
    • To determine the safety margin for intrathecal administration.

    Main Methods:

    • Administration of purified collagenase (Nucleolysin) to nucleus pulposus in dogs and monkeys.
    • Histopathological evaluation of surrounding tissues for toxicity.

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  • LD50 studies in mice and systemic toxicity studies in dogs.
  • Antigenicity testing in guinea pigs.

    • Main Results:

    • Consistent dissolution of the nucleus pulposus was observed.
    • The enzyme showed safety in surrounding local tissues, except for intrathecal administration.
    • A safety margin for intrathecal administration was indicated in monkeys.
    • Satisfactory margins of safety were shown in systemic toxicity studies.
    • No significant antigenicity was detected in guinea pigs.

    Conclusions:

    • Purified collagenase (Nucleolysin) is effective in dissolving nucleus pulposus.
    • The enzyme exhibits a favorable safety profile for local and systemic administration.
    • Further investigation into intrathecal administration safety is warranted.